Starch Lab

Krupa Desai Cell Biology April 3, 2013 Lab biogeny of Starch Introduction In this lab we learned the concept and procedure of synthesizing starch. We also learned the do of pH and temperature on the reaction rates of amylase.. In the process of the synthesis lab we learned phosphorylation victimization a potato, which was what we synthesized. The phosphorylation took place after the addition of primer. There argon two different types of starches used are amylose and amylopectin.To test for the presence of starch, the Starch Test is used. This is a simple test in which iodine is added to a given solution. If a polysaccharide such as starch is present then the iodine ion go forth lodge itself in the polysaccharide chain and give it a black-blue color. If iodine added to a solution turns black-blue than starch is present. If the solution remains the color of iodine, reddish-orange, there is no starch present, a negative test.In our experiment we descale the color we see in compariso n to the neutrals to test for the amount of starch. Methods/Materials See print out information TableBelow Time in minutes underground 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6 Tube 7 0 mins(TO) 0 0 0 0 0 ++ +++ 3 mins 0 + 0 0 + + + 6 mins + ++ + + +++ +++ +++ 9 mins 0 +++ + 0 +++ +++ ++++ 12 mins 0 +++ + 0 +++ +++ ++++ 15 mins 0 +++ + 0 ++++ ++++ ++++ 18 mins 0 +++ ++ 0 ++++ +++ ++++ 1 mins 0 +++ ++ 0 ++++ +++ +++ 24 mins 0 +++ ++ 0 +++ +++ ++++ 27 mins 0 ++++ ++ 0 +++ +++ ++++ 30 mins 0 ++++ ++ 0 ++++ ++ ++++ Discussion This lab gives a better understanding of biogeny of starch. As you can see with our data we had some discrepancies in tubes where glucose was present no starch should nominate been found however we have + in a few of the time slots this could be do to the recital of color or some spill over.Since we were trying to get exact times the drops could have spill over into another part of the dish and contaminated some of the other tubes overall our data is pretty accur ate however tube 7 had the most phosphorylation because it turned from blue to black over time. somewhat of the other tubes although had the presence of starch did not alter much over time in item most stayed the same. Additionally it is concluded the pH and temperature greatly effect the reaction rate of enzymes.